Date of Last Revision
2023-05-03 12:55:54
Major
Chemistry - Biochemistry
Degree Name
Bachelor of Science
Date of Expected Graduation
Spring 2019
Abstract
G coupled protein receptors (GCPR) are one of the largest families of receptors and mediate a variety of biological responses. Rhodopsin is the largest family and aids in sight, the α-subunit of the GCPR complex in extremely important to the activation and downstream signaling effects of GCPR. The α-subunit contains a small trans-domain portion and in this project the sequence of that portion will be inserted into a vector containing a fluorescent tag. These vectors will then be used to collect fluorescent cross correlation spectroscopy or FCCS data. The unit was cloned using assembly methods that include PCR and purification techniques. The vector containing the transducin α-subunit is then transfected into live Cos-7 cells. Images of the cell can then be taken and FCCS data can also be collected. This will give insight to the dimerization and localization of the subunit in real time live cell experiments. GCPRs are extremely important cell receptors and insights on how molecular interactions between GPCRs and other proteins can lead to new routes for drug discovery.
Research Sponsor
Dr. Adam Smith
First Reader
Dr. Leah Shriver
Second Reader
Dr. Claire Tessier
Recommended Citation
Bowman, Deanna M., "Cloning the vision related G protein transducin for live cell fluorescence studies" (2019). Williams Honors College, Honors Research Projects. 943.
https://ideaexchange.uakron.edu/honors_research_projects/943