Major

Biology

Degree Name

Bachelor of Science

Date of Graduation

Spring 2019

Abstract

The objective of this research project was to optimize a laboratory technique for visualizing primary cilia in musculoskeletal tissue of young swine, specifically the growth plate of long bones. This was accomplished through varying fixation procedures, antigen retrieval methods, antibody concentrations, and incubation times. By varying these parameters, a reproducible procedure was developed to examine the primary cilia in multiple tissues including ligament, fascia, and growth plate. This experiment introduced variances in fixation methods, with methanol or formalin. Paraffin and frozen embedding techniques were also varied for comparison with regards to cilia visualization. Results from the acetylated alpha tubulin (aTUB) stain suggested that Paraffin-embedded tissues had a significantly greater cilia / cell ratio than frozen-embedded tissues. From a qualitative standpoint, methanol-Paraffin was proposed to be the optimal treatment method for cilia detection in growth plate tissue.

Research Sponsor

Dr. Todd Blackledge

First Reader

Ashley Mohrman, Phd

Second Reader

Dr. Jordan Renna

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