Date of Last Revision
2023-05-03 12:50:18
Major
Biology
Degree Name
Bachelor of Science
Date of Expected Graduation
Spring 2019
Abstract
The objective of this research project was to optimize a laboratory technique for visualizing primary cilia in musculoskeletal tissue of young swine, specifically the growth plate of long bones. This was accomplished through varying fixation procedures, antigen retrieval methods, antibody concentrations, and incubation times. By varying these parameters, a reproducible procedure was developed to examine the primary cilia in multiple tissues including ligament, fascia, and growth plate. This experiment introduced variances in fixation methods, with methanol or formalin. Paraffin and frozen embedding techniques were also varied for comparison with regards to cilia visualization. Results from the acetylated alpha tubulin (aTUB) stain suggested that Paraffin-embedded tissues had a significantly greater cilia / cell ratio than frozen-embedded tissues. From a qualitative standpoint, methanol-Paraffin was proposed to be the optimal treatment method for cilia detection in growth plate tissue.
Research Sponsor
Dr. Todd Blackledge
First Reader
Ashley Mohrman, Phd
Second Reader
Dr. Jordan Renna
Recommended Citation
Engel, Ragen, "Optimization of Primary Cilia Detection in Musculoskeletal Tissue" (2019). Williams Honors College, Honors Research Projects. 915.
https://ideaexchange.uakron.edu/honors_research_projects/915
Included in
Animal Experimentation and Research Commons, Cell Anatomy Commons, Musculoskeletal System Commons, Orthopedics Commons