Date of Last Revision

2023-05-03 05:07:53

Major

Chemistry - Biochemistry

Degree Name

Bachelor of Science

Date of Expected Graduation

Summer 2018

Abstract

G protein coupled receptors (GPCRs) are membrane proteins with many functional roles. This family of receptors is expansive, with several subfamilies. Here, a member of subfamily A, rhodopsin (Rho) is studied. Mutations in rhodopsin have been linked to the disease retinitis pigmentosa (RP). Some of these mutations have been found to disrupt the dimerization/oligomerization of Rho at the TM4/TM5 proposed dimerization interface, causing pathogenesis of RP. In this study, two mutations of transmembrane helix 5 were investigated for changes in dimerization and stability, cell trafficking, and signaling. It was found that the V209M and F220C mutations did not result in disruption of dimerization of Rho.

Research Sponsor

Adam W. Smith

First Reader

Sailaja Paruchuri

Second Reader

Leah Shriver

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