Date of Last Revision
2023-05-03 05:08:16
Major
Chemistry - Biochemistry
Degree Name
Bachelor of Science
Date of Expected Graduation
Spring 2018
Abstract
Several glycopolymers synthesized by Activators regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP), were characterized by mass spectrometry to elucidate their microstructure and architecture. Analyses were performed using Matrix Assisted Laser Desorption Ionization- Time of Flight/ Time of Flight (MALDI-ToF/ToF) and Electrospray Ionization Mass Spectrometry (ESI-MS) instruments. The linear isopropylidenated glycopolymer (P65B1) analyzed with ESI-MS also was examined with multi-stage mass spectrometry (MSn) using collision induced dissociation (CID) to examine the tetramer parent ion. This glycopolymer was found to be either cyclic or linear (sans end groups) due to the data being fit for both forms of the glycopolymer. The CL546A1 (isopropylidene protected), and CL552a1 (acetyl protected) both showed the glycopolymer to polymerize, yet without end groups on the polymer, meaning it had cyclized. The acetyl protected polymer also showed acetyl losses in the mass spectrum. The ideal MALDI-MS conditions for this glycopolymer were for the matrix DHB, the solvent of acetonitrile (ACN), and sodium trifluoroacetate (NaTFA), which was used as a cationizing agent. In ESI-MS, ACN and NaTFA were also used. Overall synthesis of glycopolymers is known to be difficult, making characterization difficult as well, as seen in the results shown.
Research Sponsor
Dr. Chrys Wesdemiotis
First Reader
Dr. David Modarelli
Second Reader
Dr. Leah Shriver
Recommended Citation
Ganios, Maria, "Multi-dimensional Mass Spectrometry for the Characterization of Glycopolymers" (2018). Williams Honors College, Honors Research Projects. 691.
https://ideaexchange.uakron.edu/honors_research_projects/691