College
Buchtel College of Arts and Sciences
Date of Last Revision
2025-02-04 13:16:02
Major
Biomedical Science
Honors Course
BIOL 499
Number of Credits
2
Degree Name
Bachelor of Science
Date of Expected Graduation
Fall 2024
Abstract
Optimization of buffers used in phycocyanin extraction from A. marina was explored including acetate, phosphate, and citrate buffers as well as deionized water as a negative control. Samples were extracted in triplicates for each trial with two extraction trials completed overall for citrate buffer (n=6), phosphate buffer (n=6), acetate buffer (n=6), and deionized water (n=6). Results reflect no statistical difference in phycocyanin content (μg/mL) between each buffer or deionized water control. Though no statistically significant differences between treatment groups were found, phosphate buffer yielded the greatest phycocyanin content and the deionized water negative control yielded the lowest phycocyanin content overall. Further research is necessary to investigate the observed trend that phosphate buffer may produce higher quantities of phycocyanin, as results indicate no single buffer is definitively optimal for phycocyanin extraction from A. marina.
Research Sponsor
Dr. John Senko
First Reader
Isuru P. Silva Ethige
Second Reader
Angela Hartsock
Honors Faculty Advisor
Brian Bagatto
Proprietary and/or Confidential Information
No
Recommended Citation
Fleisher, Rinah, "Optimization of Buffers Used in Phycobiliprotein Extraction from the Cyanobacterium Acaryochloris marina" (2024). Williams Honors College, Honors Research Projects. 1933.
https://ideaexchange.uakron.edu/honors_research_projects/1933
