Buchtel College of Arts and Sciences

Date of Last Revision

2023-05-05 19:29:40



Honors Course

BIOL 499-002

Number of Credits


Degree Name

Bachelor of Science

Date of Expected Graduation

Spring 2023


I investigated the effects of diet and sex on zebrafish bone metabolism. Zebrafish were subjected to either a high-calorie or low-calorie diet over a five-week period. After this diet, zebrafish scales were used to measure alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) enzyme activity with a fluorescent substrate. The enzyme activities were markers of the osteoblasts (ALP) and the osteoclasts (TRAP). These data were compared among sex and diet of the zebrafish using a repeated measures ANOVA statistical test. I also measured the number of resorption lacunae per scale (another indicator of osteoclast activity). The results showed higher TRAP activity in the low-calorie diet group (p = 0.03) but no effect of sex (p = 0.55). The low-calorie diet group had a higher number of lacunae per square mm of scale (p = 0.01). I interpret these results as low-calorie treatment increases osteoclast activity in zebrafish.

Research Sponsor

Richard Londraville

First Reader

Qin Liu

Second Reader

Rachel Olson

Honors Faculty Advisor

Brian Bagatto

Proprietary and/or Confidential Information


SimonBagattoHonorThesisApprovalForm1.pdf (279 kB)
Thesis Signature Page



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