College
Buchtel College of Arts and Sciences
Date of Last Revision
2023-05-05 19:29:40
Major
Biology
Honors Course
BIOL 499-002
Number of Credits
2
Degree Name
Bachelor of Science
Date of Expected Graduation
Spring 2023
Abstract
I investigated the effects of diet and sex on zebrafish bone metabolism. Zebrafish were subjected to either a high-calorie or low-calorie diet over a five-week period. After this diet, zebrafish scales were used to measure alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) enzyme activity with a fluorescent substrate. The enzyme activities were markers of the osteoblasts (ALP) and the osteoclasts (TRAP). These data were compared among sex and diet of the zebrafish using a repeated measures ANOVA statistical test. I also measured the number of resorption lacunae per scale (another indicator of osteoclast activity). The results showed higher TRAP activity in the low-calorie diet group (p = 0.03) but no effect of sex (p = 0.55). The low-calorie diet group had a higher number of lacunae per square mm of scale (p = 0.01). I interpret these results as low-calorie treatment increases osteoclast activity in zebrafish.
Research Sponsor
Richard Londraville
First Reader
Qin Liu
Second Reader
Rachel Olson
Honors Faculty Advisor
Brian Bagatto
Proprietary and/or Confidential Information
No
Recommended Citation
Bagatto, Simon, "The Effects of Sex on Zebrafish Bone Metabolism" (2023). Williams Honors College, Honors Research Projects. 1672.
https://ideaexchange.uakron.edu/honors_research_projects/1672
Thesis Signature Page
Included in
Animal Experimentation and Research Commons, Biochemical Phenomena, Metabolism, and Nutrition Commons, Biology Commons, Cell Biology Commons, Cellular and Molecular Physiology Commons, Enzymes and Coenzymes Commons, Osteopathic Medicine and Osteopathy Commons