Quantifying Mafenide Release from 3D Printed Phenylalanine-Coumarin Copolyester Scaffolds

Author

Jacob SeehFollow

College

College of Engineering and Polymer Science

Date of Last Revision

2023-05-04 10:16:46

Major

Chemical Engineering

Honors Course

4200-497

Number of Credits

2

Degree Name

Bachelor of Science

Date of Expected Graduation

Spring 2021

Abstract

This project addressed burn wound healing through controlled release of the antibacterial drug mafenide encapsulated by a copolyster into 3D printed scaffolds. Scaffolds were printed at 25^oC and 0.77-1 bar in a cross-hatch pattern with uniform thickness, distance between parallel layers, and diameter then photo-crosslinked. The number of scaffolds available for testing was limited by the amount of polymer that could be synthesized. A high-performance liquid chromatography method was developed specifically for this experiment and used to determine daily release of mafenide from the scaffold into a 2-mL phosphate buffer solution. During the first 24 hours a large burst release of 299.9 mg or 7.9% of the initial drug amount was observed for the 2-layer scaffolds and 403.1 mg or 8.7% of its initial drug amount for the 3-layer scaffolds. Within 120 hours, the release rate had dropped to less than 100 mg per day for both scaffold types. The cumulative average release for the 2-layer and 3-layer scaffolds was determined to be 1449.0 mg (38.8% of the initial total) and 1469.5 mg (31.8% of the initial total) respectively. The results suggest that these scaffolds have the potential to become a viable treatment option in the future.

Research Sponsor

Abraham Joy

First Reader

Roya Gitiafroz

Second Reader

Bi-min Zhang Newby

Honors Faculty Advisor

Bi-min Zhang Newby

Recommended Citation

Seeh, Jacob, "Quantifying Mafenide Release from 3D Printed Phenylalanine-Coumarin Copolyester Scaffolds" (2021). Williams Honors College, Honors Research Projects. 1421.
https://ideaexchange.uakron.edu/honors_research_projects/1421