College
Buchtel College of Arts and Sciences (BCAS)
Date of Last Revision
2023-05-03 18:13:18
Major
Chemistry
Honors Course
3150:497
Number of Credits
2
Degree Name
Bachelor of Science
Date of Expected Graduation
Spring 2020
Abstract
Nonmuscle invasive bladder cancer (NMIBC) inflicts thousands of Americans annually, and is typically treated with the immunotherapy BCG. However, due to a BCG shortage, there is a new need for novel treatments of NMIBC. Addressing this issue, several imidazolium salt derivatives were synthesized and characterized with the intent of treatment within the bladder. These imidazolium salts were tested against different human bladder cancer cell lines in vitro to determine their reactivity and cytotoxicity. Among these results are GI50 concentrations for each drug, which is the concentration of drug needed to see growth inhibition in 50% of treated cells. Previous published research shows that these imidazolium salts do not target the cell nucleus, and it is believed that these drugs proceed through a mitochondrial apoptotic pathway. To show this, mitochondria were extracted from human bladder cancer cell lines and treated with drug, and detection of cytochrome c leaving the mitochondria by immunoblot (the first step in the mitochondrial apoptotic caspase-pathway) supports that these imidazolium salts proceed through a mitochondrial mechanism.
Research Sponsor
Dr. Wiley Youngs
First Reader
Dr. Philip Abbosh
Second Reader
Dr. Christopher Ziegler
Honors Faculty Advisor
Dr. Claire Tessier
Recommended Citation
Weader, David, "Synthesis, Characterization, and Biological Activity of Imidazolium Salts" (2020). Williams Honors College, Honors Research Projects. 1136.
https://ideaexchange.uakron.edu/honors_research_projects/1136
Included in
Medicinal-Pharmaceutical Chemistry Commons, Molecular Biology Commons, Organic Chemistry Commons