College
Buchtel College of Arts and Sciences (BCAS)
Date of Last Revision
2023-05-03 23:31:09
Major
Natural Sciences - BS / MD Program
Honors Course
3150:497
Number of Credits
2
Degree Name
Bachelor of Arts
Date of Expected Graduation
Spring 2020
Abstract
In this particular study global metabolomics LCMS data was analyzed to determine the effect of influenza infection on host metabolic processes. Specifically, wild-type male and female mouse lung samples from mice infected with PR8 (H1N1) or both PR8 and a reinfection of X31 (H3N2) were analyzed. The LC/MS(liquid chromatography-mass spectrometry) based global metabolomics will be performed on the samples using hydrophilic interaction liquid chromatography to separate polar analytes from the tissues (Dettmer, Aronov, 2007). Metabolites impacted by influenza infection in all samples will be analyzed to compare male and female mice, as well as differences between PR8 infection and X31 reinfection. Bioinformatic analysis will be done using MIDTOD-a software which performs automated, high throughput statistical analysis and identification of metabolites. Information regarding the regulatory and biosynthetic relationships between the metabolites and genes and proteins is also obtained. This metabolite analysis is part of a larger study being done (including genomics and proteomics) to fully characterize the effects of Influenza infection on cellular function.
Research Sponsor
Dr. Leah Shriver
First Reader
Dr. Catherine Konopka
Second Reader
Dr. Rolando Ramirez
Honors Faculty Advisor
Dr. Brian Bagatto
Recommended Citation
Mokkapati, Megha, "The Role of Influenza Infection on Cell Metabolism" (2020). Williams Honors College, Honors Research Projects. 1121.
https://ideaexchange.uakron.edu/honors_research_projects/1121
Final Honors Project
Included in
Bioinformatics Commons, Biology Commons, Other Chemistry Commons