College
Buchtel College of Arts and Sciences (BCAS)
Date of Last Revision
2023-05-03 17:32:09
Major
Biology
Honors Course
3100-499
Number of Credits
2
Degree Name
Bachelor of Science
Date of Expected Graduation
Spring 2020
Abstract
Preeclampsia is a disease associated with a vasoconstrictive phenotype due to disrupted signaling in the vascular endothelial growth factor (VEGF) relaxation pathway. The purpose of these studies is to determine the level of oxidative damage that is occurring in the placenta of the reduced uterine perfusion pressure (RUPP) rat, which serves as a common model for preeclampsia. RUPP and SHAM placenta tissue will be examined for oxidative stress as an indicator of tissue damage associated with the pathologically induced ischemia. Oxidative stress in the placental tissue will be measured by performing the following oxidative stress assays: 8-isoprostane test, TBARS assay, and SOD assay kit. In addition, it will be assessed whether this damage is reversible with uterine injection of L-tyrosine polyphosphate (LTP) nanoparticles that encode for VEGF2 receptors. We further hypothesize that improved VEGF signaling via receptor upregulation may ameliorate vascular signaling which would lead to improved utero-placental perfusion. Ultimately improved blood flow to the uterine circulation may decrease measurable levels of oxidative stress.
Research Sponsor
Dr. Rolando J.J. Ramirez
First Reader
Dr. Catherine Konopka
Second Reader
Dr. Qin Liu
Honors Faculty Advisor
Dr. Brian Bagatto
Recommended Citation
Ganios, Natalie, "Oxidative Stress Analysis of Placental Tissue in the RUPP Pregnant Rat" (2020). Williams Honors College, Honors Research Projects. 1083.
https://ideaexchange.uakron.edu/honors_research_projects/1083
Included in
Maternal and Child Health Commons, Obstetrics and Gynecology Commons, Other Physiology Commons