Major

Chemistry - Biochemistry

Degree Name

Bachelor of Science

Date of Graduation

Summer 2019

Abstract

Subarachnoid hemorrhage (SAH) is a stroke characterized by bleeding into the subarachnoid space of the brain, typically resulting in high mortality rate.8 Delayed cerebral ischemia (DCI), characterized by vasospasms induced arterial constriction, occurs in roughly one third of the surviving patients.20 The development of DCI and neurodegeneration could be linked to metabolic pathology that occurs after SAH, specifically iron induced changes in redox status. The oxidized environment induced by iron has the potential to functionally affect the ferroxidase ceruloplasmin (Cp), which is linked to neurodegeneration.15 Global LC-MS based metabolomics data revealed alterations in metabolism in the CSF at early and late timepoints after SAH as compared to controls. Early phase SAH and late phase SAH patients showed metabolic differences, however there was limited statistical significance. Specifically, the heme degradation product bilirubin (BR) and the glycolysis intermediate 3-phosphoglyceric acid were tentatively found to be down regulated in SAH. Iron toxicity was analyzed in a hemin induced model using differentiated SHSY5Y human neuroblastomas, revealing concentration-dependent cell killing and morphological changes. This data will be used as a baseline in future studies attempting to reverse iron toxicity via Cp treatment.

Research Sponsor

Leah Shriver

First Reader

Sailaja M Paruchuri

Second Reader

Michael Konopka

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