Buchtel College of Arts and Sciences
Date of Last Revision
Number of Credits
Bachelor of Science
Date of Expected Graduation
I made endospanin 1 knockout (KO) zebrafish to examine its effects on lipid and bone metabolism. Endospanin 1, or leptin receptor overlapping transcript (leprot), is a cytosolic protein linked to the protein hormone leptin that influences the trafficking of leptin receptors to the plasma membrane of cells. Genes for endospanin and tyrosinase (a pigmentation enzyme) were targeted via a microinjection of guide RNAs and CRISPR Cas9 into zebrafish embryos at 2-4 cell stages. I was able to disrupt the endospanin 1 gene (based upon the disruption of tyrosinase), but very few mutant zebrafish fully developed into adults. Only low KO mosaic zebrafish survived to early adulthood. If the assumption that endospanin 1 affects bone metabolism is supported, the results would suggest that endospanin 1’s elimination may prevent bones from properly forming during zebrafish development.
Dr. Richard Londraville
Dr. Qin Liu
Dr. Robert Joel Duff
Honors Faculty Advisor
Dr. Brian Bagatto
Proprietary and/or Confidential Information
Kittinger, Jared, "Knockout of Endospanin 1 via CRISPR in Zebrafish, Danio rerio" (2023). Williams Honors College, Honors Research Projects. 1658.
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