Buchtel College of Arts and Sciences

Date of Last Revision

2023-05-05 08:26:40


Biomedical Science

Honors Course


Number of Credits


Degree Name

Bachelor of Science

Date of Expected Graduation

Spring 2023


I made endospanin 1 knockout (KO) zebrafish to examine its effects on lipid and bone metabolism. Endospanin 1, or leptin receptor overlapping transcript (leprot), is a cytosolic protein linked to the protein hormone leptin that influences the trafficking of leptin receptors to the plasma membrane of cells. Genes for endospanin and tyrosinase (a pigmentation enzyme) were targeted via a microinjection of guide RNAs and CRISPR Cas9 into zebrafish embryos at 2-4 cell stages. I was able to disrupt the endospanin 1 gene (based upon the disruption of tyrosinase), but very few mutant zebrafish fully developed into adults. Only low KO mosaic zebrafish survived to early adulthood. If the assumption that endospanin 1 affects bone metabolism is supported, the results would suggest that endospanin 1’s elimination may prevent bones from properly forming during zebrafish development.

Research Sponsor

Dr. Richard Londraville

First Reader

Dr. Qin Liu

Second Reader

Dr. Robert Joel Duff

Honors Faculty Advisor

Dr. Brian Bagatto

Proprietary and/or Confidential Information




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