Title
Purification of Arabitol from Fermentation Broth of Debaryomyces Hansenii Using Glycerol as Substrate
Document Type
Article
Publication Date
Spring 2012
Abstract
Arabitol has many potential industrial applications and it can be produced by fermentation from glycerol, a major byproduct of biodiesel industry. This work aims at developing a new process for collecting and purifying arabitol from the broth of osmophilicDebaryomyces hansenii containing arabitol, unconsumed glycerol, and other minor impurities. RESULTS: The process developed and optimized in this study had the following steps: first, the broth supernatant was treated with 8 g L−1 activated carbon at 30°C and pH 6 for removal of colored organics and some impurities. Then glycerol was selectively removed by acetone extraction at 30°C. The acetone amount was optimized according to the measured solubility data of simulative glycerol-xylitol-acetone systems and real fermentation broth. The glycerol-removed remainder was next extracted for arabitol with butanol (90% of supernatant volume) at 90 °C. White arabitol crystals were collected from the cooled butanol extract. The overall recovery and purity of arabitol crystals were 66% and 95%, respectively.
Volume
88
Issue
8
First Page
1514
Last Page
1522
Recommended Citation
Ju, Lu-Kwang, "Purification of Arabitol from Fermentation Broth of Debaryomyces Hansenii Using Glycerol as Substrate" (2012). Chemical, Biomolecular, and Corrosion Engineering Faculty Research. 102.
https://ideaexchange.uakron.edu/chemengin_ideas/102