Date of Last Revision

2023-05-03 00:58:30

Major

Chemistry - Biochemistry

Degree Name

Bachelor of Science

Date of Expected Graduation

Summer 2017

Abstract

Chiari Malformation Type I is a neurodegenerative trait that can result from disease or from acquiring. Metabolomic analysis was done on normal pressure hydrocephalous and Chiari CSF samples using LC-MS and multiple bioinformatic programs. After analysis from multiple programs, we were able to analyze the stringency in statistical algorithms done by each program and determined qualities that are shared between programs that offer multiple details. We identified dysregulation in glucuronated metabolites in CSF of Chiari versus NPH. Using LC-MS, we established the experimental MS/MS of glucuronic acid in attempt to identify similarities in mass-to-charge features primarily identified. We could not positively identify glucuronic acid dysregulation in Chiari, yet established putatively dysregulated metabolic pathways.

Research Sponsor

Dr. Leah Shriver

First Reader

Dr. Adam Smith

Second Reader

Dr. Claire Tessier

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