Date of Last Revision
2023-05-02 14:17:32
Major
Chemistry - Biochemistry
Degree Name
Bachelor of Science
Date of Expected Graduation
Spring 2015
Abstract
Mass spectrometry is a method that uses masses to determine the structure of a compound. This is completed by first charging the compound until it is a cation or anion, and then its molecular weight can be determined by measuring a mass to charge ratio. The focus of this study was determination of sphingomyelin’s structure. The purpose of this was that sphingomyelin has been shown to play a critical role in many biological pathways and being able to identify this lipid could prove to be beneficial for preventative medicine. Utilizing electrospray ionization (ESI) Quadruple-Time-of-flight (Q-TOF) mass spectrometry on a Waters Synapt instrument and matrix-assisted laser desorption ionization (MALDI) TOF-TOF mass spectrometry on a Bruker UltraFlex III instrument, it was possible to verify the presence of a sphingolipid as well as the tail length and the presence of double bonds.
Research Sponsor
Chrys Wesdemiotis
First Reader
Thomas Leeper
Second Reader
Adam Smith
Recommended Citation
Ganios, Antonio, "Determination of the Structure of Sphingomyelin by Tandem Mass Spectrometry and Matrix - Assisted Laser Desorption/Ionization" (2015). Williams Honors College, Honors Research Projects. 164.
https://ideaexchange.uakron.edu/honors_research_projects/164