College
Buchtel College of Arts and Sciences (BCAS)
Date of Last Revision
2023-05-04 00:02:55
Major
Biology
Honors Course
3100 499
Number of Credits
3
Degree Name
Bachelor of Science
Date of Expected Graduation
Spring 2020
Abstract
Scales from adult zebrafish, Danio rerio, are an established model for studying osteoblast and osteoclast activity. In this study, scales in a cell culture were used to determine if the hormone leptin has an effect on activity of these bone remodeling scales. Alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) activities of zebrafish scales were measured and compared with and without leptin treatment. There was no statistically significant difference between leptin treatments in terms of ALP and TRAP activity. Differences between TRAP values between days 1 and 2 did look promising although they were not statistically significant. This study could provide an insight into any effect that leptin may have on bone metabolism considering the differences in TRAP activity as previously mentioned. A viable scale assay model, however, was established which can contribute to the understanding of the molecular role of leptin in bone metabolism.
Research Sponsor
Richard Londraville
First Reader
Dr. Jana Andronwoski
Second Reader
Dr. Catherine Konopka
Honors Faculty Advisor
Dr. Brian Bagatto
Recommended Citation
Bark, Elizabeth, "Leptin effects on bone metabolism" (2020). Williams Honors College, Honors Research Projects. 1120.
https://ideaexchange.uakron.edu/honors_research_projects/1120