We demonstrate quantitative biomolecule analysis using a micro coulter counter. Specific binding between antibody functionalized microparticles and target biomolecule cause large aggregates of microparticles. The micro coulter counter was employed to measure aggregation ratio, ratio of the aggregate counts to the total particle counts. Goat anti-rabbit IgG, used as a model biomarker, were tested in this paper. The experiment results showed that the aggregation ratio increases with the increasing biomolecule concentration, and the detectable concentration range from 16 to 160 ng/ml was achieved.
Miniaturized Systems for Chemistry and Life Sciences
Han, Y; Wu, H.; Liu, F.; Cheng, Gang; and Zhe, Jiang, "Novel Quantitative Macro Biomolecule Analysis Based on a Micro Coulter Counter" (2014). Mechanical Engineering Faculty Research. 980.