Chemical and Biomolecular Engineering Faculty Research

Title

Improved Detection of Rhamnolipid Production Using Agar Plates Containing Methylene Blue and Cetyl Trimethylammonium Bromide

Document Type

Article

Publication Date

Fall 2009

Abstract

Rhamnolipids, produced predominantly by Pseudomonas aeruginosa, are biosurfactants with important applications. For efficient culture screening according to rhamnolipid productivity, the method using agar plates containing methylene blue (MB) and cetyl trimethylammonium bromide (CTAB) was re-examined. An alternative set-up using a fixed underneath light source and image analysis software improved the detection of the circles formed due to complexation between anionic rhamnolipids and cationic MB/CTAB. The roles and effects of MB and CTAB concentrations and pH on the complexation phenomena are reported.

Volume

31

Issue

10

First Page

1583

Last Page

1588