Date of Graduation

Fall 2018

Document Type

Honors Research Project

Degree Name

Bachelor of Science

Major

Biology

Research Sponsor

James Holda

First Reader

Jordan Renna

Second Reader

Qin Liu

Abstract

The goal of this research is to develop a mouse model with a mutation in the CYP1B1 gene that would result in a phenotype similar to that seen in Primary Congenital Glaucoma (PCG) human patients. Using this mouse model, structural changes in eyes and upregulation of certain proteins were observed compared to a normal mouse. Examination of the cornea, iris, retina, ciliary body, and iridocorneal angle showed structural changes in CYP1B1 mice compared to wild type mice. These results were similar to those described in studies using other animal models. Furthermore, the current study confirmed earlier findings in other animal models, showing that P53 is upregulated in the cornea, retina, and iridocorneal angle of a CYP1B1 mutant compared to the wild-type. This suggests that a mutation in the CYP1B1 gene in a mouse model results in a phenotype similar to that seen in human patients with PCG. Therefore, this model could be used to investigate possible treatments for PCG.

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