Date of Last Revision
2023-05-02 19:02:44
Major
Chemistry - Biochemistry
Degree Name
Bachelor of Science
Date of Expected Graduation
Spring 2016
Abstract
Studying peptide interactions with model membranes have aided in understanding protein-lipid interactions more clearly. In this report, fluorescent small unilamellar vesicles (SUVs) of a known composition were made. Two peptides, RND1 and MARCKS, were also fluorescently labeled and added to a solution of SUVs. The interactions between these were observed via fluorescence quenching, read out with a spectrofluorimeter. In addition, two environments of buffer were tested: 1X PBS and 0.1X PBS. After obtaining data, it was concluded that MARCKS quenched the SUVs more than RND1. This could be due to the presence of more lysine amino acids in MARCKS, which have been known to play a role in membrane protein activity (Li et al., 2013). No significant changes were observed when the buffer conditions were manipulated.
Research Sponsor
Dr. Adam Smith
First Reader
Dr. Sailaja Paruchuri
Second Reader
Dr. Leah Shriver
Recommended Citation
Kordahi, Talia, "Lipid-Protein Interactions via Fluorescence Quenching in Small Unilamellar Vesicles" (2016). Williams Honors College, Honors Research Projects. 342.
https://ideaexchange.uakron.edu/honors_research_projects/342