College of Engineering and Polymer Science
Date of Last Revision
4300:497-003 Honor's Research
Number of Credits
Bachelor of Science
Date of Expected Graduation
This study investigated the viability of bioremediating rubber cryogrind using enriched indigenous bacteria. To begin the experiment, soils from three highway roadside locations in Kansas, KS 96 and West, KS 400 and 143rd, and 199th, were collected and transported to the lab to be studied. An initial soil characterization was run on the soil samples using distilled (DI) water mixture and 0.01 M CaCl2 to assess conductivity. The soils were tested to gather a baseline of the relationship between pH and conductivity and the impact of its distance from the roadside. Bacteria were isolated from the soils, mixed with rubber cryogrind, and put into a nutrient mix consisting of 2 g/L of (NH4)2SO4 (Ammonium Sulfate) and 200 mg/L of KH2PO4 (Monopotassium Phosphate) with the cryogrind as the carbon source. Five different cryogrind types were used in the experiment. Cryogrinds tested included TIP cryogrind (donated by the Tire Industry group), truck SBR (styrene-butadiene rubber) cryogrind, truck NR (natural rubber) cryogrind, Passenger tire CB (carbon black filler) cryogrind, and passenger tire Silica (silica filler) cryogrind.
Honors Faculty Advisor
Graham, Shane, "Biodegradation of Rubber Particles in Soil: Using Acclimated Bacteria Isolated from Kansas Soil to Degrade Cryogrinds in Slurry" (2022). Williams Honors College, Honors Research Projects. 1591.
Bacteriology Commons, Biochemistry Commons, Civil Engineering Commons, Environmental Engineering Commons, Environmental Microbiology and Microbial Ecology Commons, Other Civil and Environmental Engineering Commons, Other Microbiology Commons