College

Buchtel College of Arts and Sciences (BCAS)

Date of Last Revision

2020-05-07 11:28:49

Major

Natural Sciences - BS / MD Program

Honors Course

3150:497

Number of Credits

2

Degree Name

Bachelor of Arts

Date of Expected Graduation

Spring 2020

Abstract

In this particular study global metabolomics LCMS data was analyzed to determine the effect of influenza infection on host metabolic processes. Specifically, wild-type male and female mouse lung samples from mice infected with PR8 (H1N1) or both PR8 and a reinfection of X31 (H3N2) were analyzed. The LC/MS(liquid chromatography-mass spectrometry) based global metabolomics will be performed on the samples using hydrophilic interaction liquid chromatography to separate polar analytes from the tissues (Dettmer, Aronov, 2007). Metabolites impacted by influenza infection in all samples will be analyzed to compare male and female mice, as well as differences between PR8 infection and X31 reinfection. Bioinformatic analysis will be done using MIDTOD-a software which performs automated, high throughput statistical analysis and identification of metabolites. Information regarding the regulatory and biosynthetic relationships between the metabolites and genes and proteins is also obtained. This metabolite analysis is part of a larger study being done (including genomics and proteomics) to fully characterize the effects of Influenza infection on cellular function.

Research Sponsor

Dr. Leah Shriver

First Reader

Dr. Catherine Konopka

Second Reader

Dr. Rolando Ramirez

Honors Faculty Advisor

Dr. Brian Bagatto

Honors Project final_MMvLPS (1) (1).docx (457 kB)
Final Honors Project

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