College

Buchtel College of Arts and Sciences (BCAS)

Date of Last Revision

2020-05-07 11:28:41

Major

Biology

Honors Course

3100 499

Number of Credits

3

Degree Name

Bachelor of Science

Date of Expected Graduation

Spring 2020

Abstract

Scales from adult zebrafish, Danio rerio, are an established model for studying osteoblast and osteoclast activity. In this study, scales in a cell culture were used to determine if the hormone leptin has an effect on activity of these bone remodeling scales. Alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) activities of zebrafish scales were measured and compared with and without leptin treatment. There was no statistically significant difference between leptin treatments in terms of ALP and TRAP activity. Differences between TRAP values between days 1 and 2 did look promising although they were not statistically significant. This study could provide an insight into any effect that leptin may have on bone metabolism considering the differences in TRAP activity as previously mentioned. A viable scale assay model, however, was established which can contribute to the understanding of the molecular role of leptin in bone metabolism.

Research Sponsor

Richard Londraville

First Reader

Dr. Jana Andronwoski

Second Reader

Dr. Catherine Konopka

Honors Faculty Advisor

Dr. Brian Bagatto

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.