Date of Graduation

Spring 2018

Document Type

Honors Research Project

Degree Name

Bachelor of Science

Major

Chemical Engineering

Research Sponsor

Dr. Bi-min Zhang Newby

First Reader

Dr. Lingyun Liu

Second Reader

Dr. Edward Evans

Abstract

In this project, drying bacterial cellulose (BC) to maintain its structural integrity, and treating BC with organosilanes 3-aminopropyltriethoxysilane (APTES) and (3-Mercaptopropyl)trimethoxysilane (MPTMS) as well as with various concentration of gelatin to increase the cells per area was studied. Drying BC with hexamethyldisilazane (HMDS) by soaking the BC in ascending concentrations of ethanol and HMDS did not protect the structural integrity of the BC grown in lab but did for the BC grown in Dr. Siriporn Taokaew’s lab. Freeze drying also did not fully protect the structural integrity as the BC was placed in a freezer for several hours at -20oC before being placed in a freeze drier, but the samples provided by Dr. Siriporn Taokaew had, and they had been placed in liquid nitrogen instead of a freezer. BC treated with APTES solution for 60 seconds had approximately 122cells/mm2 and MPTMS had 165 cells/mm2, which was an increase from 119 cells/mm2 on the control, but not by a statistical amount. The BC treated for 30 seconds with MPTMS only had 175 cells/mm2, less than the control of 242 cells/mm2, the APTES had statistically more with 373 cells/mm2.

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