Date of Graduation

Spring 2016

Document Type

Honors Research Project

Degree Name

Bachelor of Science

Major

Biology

Research Sponsor

Randy Mitchell

First Reader

Jordan Renna

Second Reader

Donald Ott

Abstract

In angiosperm plants, pollen is produced in the anthers and dispersed by pollinators to the stigmas of either the same plant or other flowers. After pollen reaches the stigma, pollen tubes grow down the style transporting the male gametes, cytoplasm, and a vegetative nucleus to the ovary, which fertilizes the flower. Currently there are many different techniques to stain and study pollen tubes in order to investigate reproductive techniques of many angiosperms. Though many techniques are available, few result in images that facilitate counting of pollen tubes at different stages of pollination. In this study, a number of promising techniques for fixing, softening, and staining stigmas were compared to determine the most efficient way of viewing the numerous pollen tubes in terms of time, cost, and images produced, and therefore learn more about the reproduction process in angiosperms

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