Date of Graduation
Honors Research Project
Bachelor of Science
Chemical Engineering - Cooperative Education
The main purpose of the first part in the lab was to try and determine a way to measure the moles of dye per mole of protein in order to detect an azide tag on a recombinantly produced azide-IFN-γ protein. This was done by using click chemistry to bind the azide tagged protein to a dye and then measured the azide tag efficiency. The main purpose of the second part of the project was to make a protein, SDF-1α, and tag it with an azide group. This was completed via processes of expression, isolation, and purification of the protein. From here, an immobilization experiment was performed and a measure of the processes efficiency was recorded.
A definite conclusion can be drawn that engineering a protein, expressing it, and purifying it can be completed. A conclusion that can be made from the first part of the experiment is to find a new method to move forward with when trying to measure the amount of azide that was tagged to the protein. No conclusions could be drawn from the second part, involving the immobilization process, due to an error in the kit used in the lab.
Kraley, James P., "Click Chemistry Protein Immobilization" (2015). Honors Research Projects. 113.