Chemical and Biomolecular Engineering Faculty Research

Title

Enhancement of the Sensitivity of Surface Pasmon Resonance Biosensor with Colloidal Gold Labeling Technique

Document Type

Article

Publication Date

Fall 1998

Abstract

In our experiment colloidal gold was used to enhance the signal obtained from the surface-plasmonresonance biosensor. Fab′ fragments of human IgG molecules were immobilized on the gold surface and reacted with the sheep-anti-human IgG(SAH-IgG) molecules in solution. It was found that when the colloidal gold labeling technique was introduced the sensitivity of surface plasmon resonance instruments increased by a factor of about 300.

Volume

5

Issue

6

First Page

695

Last Page

698