Chemical and Biomolecular Engineering Faculty Research

Title

Use of Perfluorocarbon Emulsions in Cell Culture.

Document Type

Article

Publication Date

Fall 1992

Abstract

Perfluorocarbon emulsions were applied to hybridoma cultures grown in tissue culture tubes and column bioreactors. The oxygen transfer enhancement effect of perfluorocarbon emulsions was clearly demonstrated by the higher cell densities obtained in emulsion-supplemented systems. In addition, perfluorocarbon emulsions were shown to provide better cell suspension in a low-shear environment. The study in column bioreactors also suggested a cell protective effect of the employed perfluorocarbon emulsions in reducing the damage to cells by gas bubbles.

Volume

12

Issue

2

First Page

258

Last Page

263